Han, Huijong, Ph.D.

Personal Information vCard

• E-mail : huijong.han@gmail.com
• Homepage : www.zannavi.com/huijong/ (this page)
• Spouse: Son, Sang-Kil

Education

Aug. 2003 ~ Sep. 2008	Ph. D. in Medicinal Chemistry, University of Kansas, United States (Research Assistantship) Thesis: The Application of X-ray Crystallography Towards the Design of Novel Inhibitors of MurA and CDK2, Ph.D. Thesis, University of Kansas (2008) M. S. in Medicinal Chemistry, University of Kansas, United States Advisor: Prof. Ernst Schönbrunn Cloning, mutation, and purification of enolpyruvyl transferases and CDK2 High-throughput screening of enolpyruvyl transferases Inhibitor characterizations using enzymatic assays and structural studies Protein crystal screening with a crystallization robot, crystallization, X-ray data collection, and structure refinements New inhibitor design and identification by in silico screening
Mar. 1997 ~ Feb. 1999	M. S. in Chemistry, KAIST, South Korea (Full Scholarship from Government) Thesis: NMR study of catalytic DNA, M.S. Thesis, KAIST (1999) Advisor: Prof. Byong-Seok Choi DNA synthesis and purification using DNA synthesizer and HPLC Structure determination of DNAs by NMR spectroscopy
Mar. 1993 ~ Feb. 1997	B. S. in Chemistry, KAIST, South Korea (Full Scholarship from Government for 4 years)

Working Experience

Jan. 2023 ~	Sample Environment and Characterization group, European XFEL, Operations Leader User Laboratories
Jul. 2019 ~	Sample Environment and Characterization group, European XFEL, Scientist
Jan. 2016 ~ Jul. 2019	Faculty of biochemistry and molecular medicine, University of Oulu, Post-Doctoral Fellow Advisor: Prof. Inari Kursula The XFEL Biology Infrastructure (XBI), European XFEL, Guest Scientist
Nov. 2011 ~ Jul. 2015	Department of Biochemistry, University of Oulu at Deutsches Elektronen-Synchrotron (DESY), Post-Doctoral Fellow Advisor: Prof. Petri Kursula
Oct. 2008 ~ Feb. 2009	H. Lee Moffitt Cancer Center, Post-Doctoral Fellow
Oct. 2007 ~ Sep. 2008	H. Lee Moffitt Cancer Center, Research Associate Characterization of enzymes by kinetic assays Crystallization, X-ray crystallographic data collection, and structure refinements New inhibitor design and identification by in silico screening and docking
Sep. 2000 ~ Mar. 2002	CrystalGenomics, Inc., Research Scientist Drug Discovery by molecular modelling Set up target-specific compound library Analysis of protein family structures
Apr. 1999 ~ Aug. 2000	Biotech Research Institute, LG Chem. Ltd., Research Scientist Drug Discovery using SAR by NMR spectroscopy Peptide structure determination using NMR spectroscopy

Selected publications

PNAS 103, 13010 (2006)

Biochemistry 49, 4276 (2010)

J. Biol. Chem. 289, 14121 (2014)

J. Biol. Chem. 290, 3612 (2015)

Cell Host Microbe 20, 731 (2016)

Publications | Listed with thumbnails | Grouped by journals — Search in [ Google Scholar | ResearchGate | ResearcherID | ORCID ]

49. Adam Round, Piere Aller, Richard J. Bean, Johan Bielecki, Agata Butryn, et al., The single-particle, clusters and biomolecules and serial femtosecond crystallography instrument of the European x-ray free-electron laser: interaction region downstream at atmospheric pressure (IRD), J. Synchrotron Radiat. 33, 198–206 (2026)
48. Diandra Doppler, Alice Grieco, Domin Koh, Abhik Manna, Adil Ansari, et al., Minimized sample consumption for time-resolved serial crystallography applied to the redox cycle of human NQO1, Commun. Chem. 9, 107 (2026)
47. Mohammad Vakili, Saša Bajt, Johan Bielecki, Sabine Botha, Claire Cerniglia, et al., PEO-sheathed liquid jets increase sample delivery stability for serial femtosecond X-ray crystallography, Sci. Rep. 16, 10497 (2026)
46. Mariya Aleksich, Yeongsu Cho, Daniel W. Paley, Maggie C. Willson, Hawi N. Nyiera, et al., Ligand-mediated quantum yield enhancement in 1-D silver organothiolate metal–organic chalcogenolates, Adv. Funct. Mater. 35, 2414914 (2025)
45. Soshichiro Nagano, David von Stetten, Kaoling Guan, Peng-Yuan Chen, Chen Song, et al., Pr and Pfr structures of plant phytochrome A, Nat. Commun. 16, 5319 (2025)
44. Christina Schmidt, Kristina Lorenzen, Joachim Schulz, and Huijong Han, Standard Sample Preparation for Serial Femtosecond Crystallography, Biomolecules 15, 1488 (2025)
43. Dmitrii Zabelskii, Ekaterina Round, Huijong Han, David von Stetten, Romain Letrun, et al., Viscoelastic characterization of the lipid cubic phase provides insights into high-viscosity extrusion injection for XFEL experiments, Sci. Rep. 15, 38999 (2025)
42. Anaïs Chretien, Marius F. Nagel, Sabine Botha, Raphaël de Wijn, Lea Brings, et al., Light-induced Trp_in/Met_out Switching During BLUF Domain Activation in ATP-bound Photoactivatable Adenylate Cyclase OaPAC, J. Mol. Biol. 436, 168439 (2024)
41. Samuel Perrett, Alisia Fadini, Christopher D.M. Hutchison, Sayantan Bhattacharya, Cade Morrison, et al., Kilohertz droplet-on-demand serial femtosecond crystallography at the European XFEL station FXE, Struct. Dyn. 11, 024310 (2024)
40. Patrick Y.A. Reinke, Robin Schubert, Dominik Oberthür, Marina Galchenkova, Aida Rahmani Mashhour, et al., SARS-CoV-2 M^pro responds to oxidation by forming disulfide and NOS/SONOS bonds, Nat. Commun. 15, 3827 (2024)
39. Faisal H.M. Koua, Huijong Han, and Richard Bean, Structural Dynamics of Metalloproteins and Redox Enzymology with Mix-and-Inject Time-Resolved Serial Femtosecond Crystallography, ACS Catal. 14, 10853–10870 (2024)
38. Andrea Moreno, Isabel Quereda-Moraleda, Celia Lozano-Vallhonrat, María Buñuel-Escudero, Sabine Botha, et al., New insights into the function and molecular mechanisms of Ferredoxin-NADP⁺ reductase from Brucella ovis, Arch. Biochem. Biophys. 762, 110204 (2024)
37. Sofia M. Kapetanaki, Nicolas Coquelle, David von Stetten, Martin Byrdin, Ronald Rios-Santacruz, et al., Crystal structure of a bacterial photoactivated adenylate cyclase determined by serial femtosecond and serial synchrotron crystallography, IUCrJ 11, 991–1006 (2024)
36. Mohammad Vakili, Huijong Han, Christina Schmidt, Agnieszka Wrona, Marco Kloos, et al., Mix-and-extrude: high-viscosity sample injection towards time-resolved protein crystallography, J. Appl. Cryst. 56, 1038–1045 (2023)
35. Özlem Kalkan, Sravya Kantamneni, Lea Brings, Huijong Han, Richard Bean, et al., Heterologous expression, purification and structural features of native Dictyostelium discoideum dye-decolorizing peroxidase bound to a natively incorporated heme, Front. Chem. 11, 1220543 (2023)
34. Mariya Aleksich, Daniel W. Paley, Elyse A. Schriber, Will Linthicum, Vanessa Oklejas, et al., XFEL Microcrystallography of Self-Assembling Silver n-Alkanethiolates, J. Am. Chem. Soc. 145, 17042–17055 (2023)
33. F. A. Lima, F. Otte, M. Vakili, F. Ardana-Lamas, M. Biednov, et al., Experimental capabilities for liquid jet samples at sub-MHz rates at the FXE Instrument at European XFEL, J. Synchrotron Radiat. 30, 1168–1182 (2023)
32. Marcin Sikorski, Marco Ramilli, Raphael de Wijn, Viktoria Hinger, Aldo Mozzanica, et al., First operation of the JUNGFRAU detector in 16-memory cell mode at European XFEL, Front. Phys. 11, 1303247 (2023)
31. Vasundara Srinivasan, Hévila Brognaro, Prince R. Prabhu, Edmarcia Elisa de Souza, Sebastian Günther, et al., Antiviral activity of natural phenolic compounds in complex at an allosteric site of SARS-CoV-2 papain-like protease, Commun. Biol. 5, 805 (2022)
30. Wiebke Ewert, Sebastian Günther, Francesca Miglioli, Sven Falke, Patrick Y.A. Reinke, et al., Hydrazones and thiosemicarbazones targeting protein-protein-interactions of SARS-CoV-2 papain-like protease, Front. Chem. 10, 832431 (2022)
29. Mohammad Vakili, Johan Bielecki, Juraj Knoška, Florian Otte, Huijong Han, et al., 3D printed devices and infrastructure for liquid sample delivery at the European XFEL, J. Synchrotron Radiat. 29, 331–346 (2022)
28. Mukul Sonker, Diandra Doppler, Ana Egatz-Gomez, Sahba Zaare, Mohammad T. Rabbani, et al., Electrically stimulated droplet injector for reduced sample consumption in serial crystallography, Biophys. Rep. 2, 100081 (2022)
27. Sebastian Günther, Patrick Y.A. Reinke, Yaiza Fernández-García, Julia Lieske, Thomas J. Lane, et al., X-ray screening identifies active site and allosteric inhibitors of SARS-CoV-2 main protease, Science 372, 642–646 (2021)
26. Huijong Han, Ekaterina Round, Robin Schubert, Yasmin Gül, Jana Makroczyová, et al., The XBI BioLab for life science experiments at the European XFEL, J. Appl. Cryst. 54, 7–21 (2021)
25. Sebastian Günther, Patrick Y.A. Reinke, Dominik Oberthuer, Oleksandr Yefanov, Helen Ginn, et al., Catalytic cleavage of HEAT and subsequent covalent binding of the tetralone moiety by the SARS-CoV-2 main protease, (preprint, 2020)
24. Arne Raasakka, Salla Ruskamo, Julia Kowal, Huijong Han, Anne Baumann, et al., Molecular structure and function of myelin protein P0 in membrane stacking, Sci. Rep. 9, 642 (2019)
23. Esa-Pekka Kumpula, Andrea J. Lopez, Leila Tajedin, Huijong Han, and Inari Kursula, Atomic view into Plasmodium actin polymerization, ATP hydrolysis, and fragmentation, PLoS Biol. 17, e3000315 (2019)
22. Huijong Han and Petri Kursula, Crystallographic anomalous diffraction data for the experimental phasing of two myelin proteins, gliomedin and periaxin, Data in Brief 11, 552–556 (2017)
21. Damien Jacot, Nicolò Tosetti, Isa Pires, Jessica Stock, Arnault Graindorge, et al., An Apicomplexan Actin-Binding Protein Serves as a Connector and Lipid Sensor to Coordinate Motility and Invasion, Cell Host Microbe 20, 731–743 (2016)
20. Huijong Han and Petri Kursula, The Olfactomedin Domain from Gliomedin is a β-Propeller with Unique Structural Properties, J. Biol. Chem. 290, 3612–3621 (2015)
19. Huijong Han and Petri Kursula, Periaxin and AHNAK nucleoprotein 2 form intertwined homodimers through domain swapping, J. Biol. Chem. 289, 14121–14131 (2014)
18. Huijong Han and Petri Kursula, Expression, purification, crystallization and preliminary X-ray crystallographic analysis of the extracellular olfactomedin domain of gliomedin, Acta Cryst. F 70, 1536–1539 (2014)
17. Huijong Han, Matti Myllykoski, Salla Ruskamo, Chaozhan Wang, and Petri Kursula, Myelin-specific proteins: a structurally diverse group of membrane-interacting molecules, BioFactors 39, 233–241 (2013)
16. Huijong Han and Petri Kursula, Preliminary crystallographic analysis of the N-terminal PDZ-like domain of periaxin, an abundant peripheral nerve protein linked to human neuropathies, Acta Cryst. F 69, 804–808 (2013)
15. Ernst Schonbrunn, Stephane Betzi, Riazul Alam, Martin P. Martin, Andreas Becker, et al., Development of highly potent and selective diaminothiazole inhibitors of cyclin-dependent kinases, J. Med. Chem. 56, 3768–3782 (2013)
14. Matti Myllykoski, Arne Raasakka, Mari Lehtimäki, Huijong Han, Inari Kursula, et al., Crystallographic analysis of the reaction cycle of 2',3'-cyclic nucleotide 3'-phosphodiesterase, a unique member of the 2H phosphoesterase family, J. Mol. Biol. 425, 4307–4322 (2013)
13. Matti Myllykoski, Arne Raasakka, Huijong Han, and Petri Kursula, Myelin 2',3'-cyclic nucleotide 3'-phosphodiesterase: Active-site ligand binding and molecular conformation, PLoS ONE 7, e32336 (2012)
12. Jin-Yi Zhu, Yan Yang, Huijong Han, Stephane Betzi, Sanne H. Olesen, et al., Functional consequence of covalent reaction of phosphoenolpyruvate with UDP-N-acetylglucosamine 1-carboxyvinyltransferase (MurA), J. Biol. Chem. 287, 12657–12667 (2012)
11. Stephane Betzi, Riazul Alam, Mathew Martin, Donna J. Lubbers, Huijong Han, et al., Discovery of a potential allosteric ligand binding site in CDK2, ACS Chem. Biol. 6, 492–501 (2011)
10. Huijong Han, Yan Yang, Sanne H. Olesen, Andreas Becker, Stephane Betzi, et al., The Fungal Product Terreic Acid is a Covalent Inhibitor of the Bacterial Cell Wall Biosynthetic Enzyme UDP-N-acetylglucosamine 1-carboxyvinyltransferase (MurA), Biochemistry 49, 4276–4282 (2010)
9. Todd Funke, Yan Yang, Huijong Han, Martha Healy-Fried, Sanne Olesen, et al., Structural Basis of Glyphosate Resistance Resulting from the Double Mutation Thr⁹⁷ → Ile and Pro¹⁰¹ → Ser in 5-Enolpyruvylshikimate-3-phosphate Synthase from Escherichia coli, J. Biol. Chem. 284, 9854–9860 (2009)
8. An Wang, Juan Carlos Rodríguez, Huijong Han, Ernst Schönbrunn, and Mario Rivera, X-ray Crystallographic and Solution State Nuclear Magnetic Resonance Spectroscopic Investigations of NADP⁺ Binding to Ferredoxin NADP Reductase from Pseudomonas aeruginosa, Biochemistry 47, 8080–8093 (2008)
7. Todd Funke, Martha L. Healy-Fried, Huijong Han, David G. Alberg, Paul A. Bartlett, et al., Differential Inhibition of Class I and Class II 5-Enolpyruvylshikimate-3-phosphate Synthases by Tetrahedral Reaction Intermediate Analogues, Biochemistry 46, 13344–13351 (2007)
6. An Wang, Yuhong Zeng, Huijong Han, Saroja Weeratunga, Bailey N. Morgan, et al., Biochemical and Structural Characterization of Pseudomonas aeruginosa Bfd and FPR: Ferredoxin NADP⁺ Reductase and not Ferredoxin is the Redox Partrner of Heme Oxygenase under Iron-Starvation Conditions, Biochemistry 46, 12198–12211 (2007)
5. Martha L. Healy-Fried, Todd Funke, Melanie A. Priestman, Huijong Han, and Ernst Schönbrunn, Structural Basis of Glyphosate Tolerance Resulting from Mutations of Pro¹⁰¹ in Escherichia coli 5-Enolpyruvylshikimate-3-phosphate Synthase, J. Biol. Chem. 282, 32949–32955 (2007)
4. Todd Funke, Huijong Han, Martha L. Healy-Fried, Markus Fischer, and Ernst Schönbrunn, Molecular basis for the herbicide resistance of Roundup Ready crops, Proc. Natl. Acad. Sci. U. S. A. 103, 13010–13015 (2006)
3. Xiao Ming Yu, Huijong Han, and Brian S.J. Blagg, Synthesis of Mono- and Dihydroxylated Furanoses, Pyranoses, and an Oxepanose for the Preparation of Natural Product Analogue Libraries, J. Org. Chem. 70, 5599–5605 (2005)
2. Yun-Jeong Choi, Hui-Jong Han, Joon-Hwa Lee, Se-Won Suh, and Byong-Seok Choi, Synthesis and NMR Studies of the RNA-Cleaving DNA Enzyme, Bull. Korean Chem. Soc. 21, 955–956 (2000)
1. Joon-Hwa Lee, Jin-Young Park, Hi-Jung Han, and Byong-Seok Choi, Conformational Study of Decamer DNA Duplex d(ACGTATACGT)₂ by NMR Spectroscopy, J. Kor. Magn. Reson. Soc. 1, 63–70 (1997)

Abstracts

7. Huijong Han, Lea Brings, Anaïs Chretien, Iñaki de Diego, Ekaterina Round, Christina Schmidt, Robin Schubert, Joachim Schulz, and Kristina Lorenzen, Protein crystallization and characterization for serial femtosecond crystallography at the European XFEL in 33rd European Crystallography Meeting (Versailles, France, August 23-27, 2022)
6. Huijong Han, Yasmin Guel, Inari Kursula, Jana Makroczyova, Ekaterina Round, Robin Schubert, Joachim Schulz, and Kristina Lorenzen, Sample preparation and characterization for serial femtosecond crystallography at European XFEL in 16th Conference of the Asian Crystallographic Association (National University of Singapore, Singapore, December 17-20, 2019)
5. Huijong Han and Ernst Schönbrunn, Molecular Modes of Action of Novel CDK2 Inhibitors in The 45th Annual MIKI Meeting (University of Kansas, Lawrence, Kansas, Apr. 13-15, 2007) [oral presentation]
4. Huijong Han, Todd Funke, Melanie Priestman, and Ernst Schönbrunn, Discovery of Novel MurA Inhibitors in The 6th Biennial GPEN Conference (University of Kansas, Lawrence, Kansas, Oct. 25-27, 2006)
3. Huijong Han, Todd Funke, Melanie Priestman, and Ernst Schönbrunn, Discovery and Evaluation of Novel MurA Inhibitors in Structure Based Drug Discovery (D6) (Keystone Symposia, Whistler, British Columbia, Canada, Apr. 4-9, 2006)
2. Huijong Han, Joon-Hwa Lee, and Byong-Seok Choi, The Solution Structure of DNA Decamer Duplex Containing the Dewar Product of the Thymydylyl(3’–5’)thymidine by the NMR Spectroscopy and the Full Relaxation Matrix Refinement in Abstract of the 82nd Annual Meeting of the Korean Chemical Society (Oct. 23-24, 1998)
1. Huijong Han, Joon-Hwa Lee, and Byong-Seok Choi, The Solution Structure of DNA Decamer Duplex Containing the Dewar Product of the Thymydylyl(3’–5’)thymidine by the NMR Spectroscopy and the Full Relaxation Matrix Refinement in XVIII International Conference on Magnetic Resonance in Biological Systems (Tokyo Metropolitan University, Tokyo, Japan, August 1998)

Awards

• Goetsch Family Scholarship
• Full governmental scholarship for undergraduate and graduate students

bibTeX | pdf | slide pdf | poster pdf | abstract | external link