Han, Huijong, Ph.D.

Scientist, European XFEL
Holzkoppel 4
22869 Schenefeld, Germany

Personal Information vCard
Aug. 2003 ~ Sep. 2008    Ph. D. in Medicinal Chemistry, University of Kansas, United States (Research Assistantship)
Thesis: The Application of X-ray Crystallography Towards the Design of Novel Inhibitors of MurA and CDK2, Ph.D. Thesis, University of Kansas (2008) [bib][pdf][abstract]
M. S. in Medicinal Chemistry, University of Kansas, United States
Advisor: Prof. Ernst Schönbrunn
  • Cloning, mutation, and purification of enolpyruvyl transferases and CDK2
  • High-throughput screening of enolpyruvyl transferases
  • Inhibitor characterizations using enzymatic assays and structural studies
  • Protein crystal screening with a crystallization robot, crystallization, X-ray data collection, and structure refinements
  • New inhibitor design and identification by in silico screening
Mar. 1997 ~ Feb. 1999    M. S. in Chemistry, KAIST, South Korea (Full Scholarship from Government)
Thesis: NMR study of catalytic DNA, M.S. Thesis, KAIST (1999) [bib][abstract]
Advisor: Prof. Byong-Seok Choi
  • DNA synthesis and purification using DNA synthesizer and HPLC
  • Structure determination of DNAs by NMR spectroscopy
Mar. 1993 ~ Feb. 1997    B. S. in Chemistry, KAIST, South Korea (Full Scholarship from Government for 4 years)

Working Experience
Jul. 2019 ~    Sample Environment and Characterization group, European XFEL, Scientist
Jan. 2016 ~ Jul. 2019    Faculty of biochemistry and molecular medicine, University of Oulu, Post-Doctoral Fellow
Advisor: Prof. Inari Kursula
The XFEL Biology Infrastructure (XBI), European XFEL, Guest Scientist
Nov. 2011 ~ Jul. 2015    Department of Biochemistry, University of Oulu at Deutsches Elektronen-Synchrotron (DESY), Post-Doctoral Fellow
Advisor: Prof. Petri Kursula
Oct. 2008 ~ Feb. 2009    H. Lee Moffitt Cancer Center, Post-Doctoral Fellow
Oct. 2007 ~ Sep. 2008    H. Lee Moffitt Cancer Center, Research Associate
  • Characterization of enzymes by kinetic assays
  • Crystallization, X-ray crystallographic data collection, and structure refinements
  • New inhibitor design and identification by in silico screening and docking
Sep. 2000 ~ Mar. 2002    CrystalGenomics, Inc., Research Scientist
  • Drug Discovery by molecular modelling
  • Set up target-specific compound library
  • Analysis of protein family structures
Apr. 1999 ~ Aug. 2000    Biotech Research Institute, LG Chem. Ltd., Research Scientist
  • Drug Discovery using SAR by NMR spectroscopy
  • Peptide structure determination using NMR spectroscopy

Selected publications
PNAS 103, 13010 (2006)
Biochemistry 49, 4276 (2010)
J. Biol. Chem. 289, 14121 (2014)
J. Biol. Chem. 290, 3612 (2015)
Cell Host Microbe 20, 731 (2016)

  1. Joon-Hwa Lee, Jin-Young Park, Hi-Jung Han, and Byong-Seok Choi, Conformational Study of Decamer DNA Duplex d(ACGTATACGT)2 by NMR Spectroscopy, J. Kor. Magn. Reson. Soc. 1, 63–70 (1997) [bib][pdf][abstract][link]
  2. Yun-Jeong Choi, Hui-Jong Han, Joon-Hwa Lee, Se-Won Suh, and Byong-Seok Choi, Synthesis and NMR Studies of the RNA-Cleaving DNA Enzyme, Bull. Korean Chem. Soc. 21, 955–956 (2000) [bib][pdf][link]
  3. Xiao Ming Yu, Huijong Han, and Brian S.J. Blagg, Synthesis of Mono- and Dihydroxylated Furanoses, Pyranoses, and an Oxepanose for the Preparation of Natural Product Analogue Libraries, J. Org. Chem. 70, 5599–5605 (2005) [bib][abstract][link]
  4. Todd Funke, Huijong Han, Martha L. Healy-Fried, Markus Fischer, and Ernst Schönbrunn, Molecular basis for the herbicide resistance of Roundup Ready crops, Proc. Natl. Acad. Sci. U. S. A. 103, 13010–13015 (2006) [bib][pdf][abstract][link]
  5. Todd Funke, Martha L. Healy-Fried, Huijong Han, David G. Alberg, Paul A. Bartlett, and Ernst Schönbrunn, Differential Inhibition of Class I and Class II 5-Enolpyruvylshikimate-3-phosphate Synthases by Tetrahedral Reaction Intermediate Analogues, Biochemistry 46, 13344–13351 (2007) [bib][abstract][link]
  6. Martha L. Healy-Fried, Todd Funke, Melanie A. Priestman, Huijong Han, and Ernst Schönbrunn, Structural Basis of Glyphosate Tolerance Resulting from Mutations of Pro101 in Escherichia coli 5-Enolpyruvylshikimate-3-phosphate Synthase, J. Biol. Chem. 282, 32949–32955 (2007) [bib][pdf][abstract][link]
  7. An Wang, Yuhong Zeng, Huijong Han, Saroja Weeratunga, Bailey N. Morgan, Pierre Moënne-Loccoz, Ernst Schönbrunn, and Mario Rivera, Biochemical and Structural Characterization of Pseudomonas aeruginosa Bfd and FPR: Ferredoxin NADP+ Reductase and not Ferredoxin is the Redox Partrner of Heme Oxygenase under Iron-Starvation Conditions, Biochemistry 46, 12198–12211 (2007) [bib][abstract][link]
  8. An Wang, Juan Carlos Rodríguez, Huijong Han, Ernst Schönbrunn, and Mario Rivera, X-ray Crystallographic and Solution State Nuclear Magnetic Resonance Spectroscopic Investigations of NADP+ Binding to Ferredoxin NADP Reductase from Pseudomonas aeruginosa, Biochemistry 47, 8080–8093 (2008) [bib][abstract][link]
  9. Todd Funke, Yan Yang, Huijong Han, Martha Healy-Fried, Sanne Olesen, Andreas Becker, and Ernst Schönbrunn, Structural Basis of Glyphosate Resistance Resulting from the Double Mutation Thr97 → Ile and Pro101 → Ser in 5-Enolpyruvylshikimate-3-phosphate Synthase from Escherichia coli, J. Biol. Chem. 284, 9854–9860 (2009) [bib][pdf][abstract][link]
  10. Huijong Han, Yan Yang, Sanne H. Olesen, Andreas Becker, Stephane Betzi, and Ernst Schönbrunn, The Fungal Product Terreic Acid is a Covalent Inhibitor of the Bacterial Cell Wall Biosynthetic Enzyme UDP-N-acetylglucosamine 1-carboxyvinyltransferase (MurA), Biochemistry 49, 4276–4282 (2010) [bib][abstract][link]
  11. Stephane Betzi, Riazul Alam, Mathew Martin, Donna J. Lubbers, Huijong Han, Sudhakar R. Jakkaraj, Gunda I. Georg, and Ernst Schönbrunn, Discovery of a potential allosteric ligand binding site in CDK2, ACS Chem. Biol. 6, 492–501 (2011) [bib][abstract][link]
  12. Matti Myllykoski, Arne Raasakka, Huijong Han, and Petri Kursula, Myelin 2',3'-cyclic nucleotide 3'-phosphodiesterase: Active-site ligand binding and molecular conformation, PLoS ONE 7, e32336 (2012) [bib][pdf][abstract][link]
  13. Jin-Yi Zhu, Yan Yang, Huijong Han, Stephane Betzi, Sanne H. Olesen, Frank Marsilio, and Ernst Schönbrunn, Functional consequence of covalent reaction of phosphoenolpyruvate with UDP-N-acetylglucosamine 1-carboxyvinyltransferase (MurA), J. Biol. Chem. 287, 12657–12667 (2012) [bib][pdf][abstract][link]
  14. Huijong Han, Matti Myllykoski, Salla Ruskamo, Chaozhan Wang, and Petri Kursula, Myelin-specific proteins: a structurally diverse group of membrane-interacting molecules, BioFactors 39, 233–241 (2013) [bib][abstract][link]
  15. Ernst Schonbrunn, Stephane Betzi, Riazul Alam, Martin P. Martin, Andreas Becker, Huijong Han, Rawle Francis, Ramappa Chakrasali, Sudhakar R. Jakkaraj, Aslamuzzaman Kazi, Said M. Sebti, Christopher L. Cubitt, Anthony W. Gebhard, Lori Hazlehurst, Joseph Sherwin Tash, and Gunda I. Georg, Development of highly potent and selective diaminothiazole inhibitors of cyclin-dependent kinases, J. Med. Chem. 56, 3768–3782 (2013) [bib][abstract][link]
  16. Huijong Han and Petri Kursula, Preliminary crystallographic analysis of the N-terminal PDZ-like domain of periaxin, an abundant peripheral nerve protein linked to human neuropathies, Acta Cryst. F 69, 804–808 (2013) [bib][pdf][abstract][link]
  17. Matti Myllykoski, Arne Raasakka, Mari Lehtimäki, Huijong Han, Inari Kursula, and Petri Kursula, Crystallographic analysis of the reaction cycle of 2',3'-cyclic nucleotide 3'-phosphodiesterase, a unique member of the 2H phosphoesterase family, J. Mol. Biol. 425, 4307–4322 (2013) [bib][abstract][link]
  18. Huijong Han and Petri Kursula, Periaxin and AHNAK nucleoprotein 2 form intertwined homodimers through domain swapping, J. Biol. Chem. 289, 14121–14131 (2014) [bib][pdf][abstract][link]
  19. Huijong Han and Petri Kursula, Expression, purification, crystallization and preliminary X-ray crystallographic analysis of the extracellular olfactomedin domain of gliomedin, Acta Cryst. F 70, 1536–1539 (2014) [bib][pdf][abstract][link]
  20. Huijong Han and Petri Kursula, The Olfactomedin Domain from Gliomedin is a β-Propeller with Unique Structural Properties, J. Biol. Chem. 290, 3612–3621 (2015) [bib][pdf][abstract][link]
  21. Damien Jacot, Nicolò Tosetti, Isa Pires, Jessica Stock, Arnault Graindorge, Yu-Fu Hung, Huijong Han, Rita Tewari, Inari Kursula, and Dominique Soldati-Favre, An Apicomplexan Actin-Binding Protein Serves as a Connector and Lipid Sensor to Coordinate Motility and Invasion, Cell Host Microbe 20, 731–743 (2016) [bib][abstract][link]
  22. Huijong Han and Petri Kursula, Crystallographic anomalous diffraction data for the experimental phasing of two myelin proteins, gliomedin and periaxin, Data in Brief 11, 552–556 (2017) [bib][pdf][abstract][link]
  23. Arne Raasakka, Salla Ruskamo, Julia Kowal, Huijong Han, Anne Baumann, Anna Fasano Matti Myllykoski, Rocco Rossano, Paolo Riccio, Jochen Bürck, Anne S. Ulrich, Henning Stahlberg, and Petri Kursula, Molecular structure and function of myelin protein P0 in membrane stacking, Sci. Rep. 9, 642 (2019) [bib][pdf][abstract][link]
  24. Esa-Pekka Kumpula, Andrea J. Lopez, Leila Tajedin, Huijong Han, and Inari Kursula, Atomic view into Plasmodium actin polymerization, ATP hydrolysis, and fragmentation, PLoS Biol. 17, e3000315 (2019) [bib][pdf][abstract][link]
  25. Sebastian Günther, Patrick Y.A. Reinke, Dominik Oberthuer, Oleksandr Yefanov, Helen Ginn, Susanne Meier, Thomas J. Lane, Kristina Lorenzen, Luca Gelisio, Wolfgang Brehm, Illona Dunkel, Martin Domaracky, Sofiane Saouane, Julia Lieske, Christiane Ehrt, Faisal Koua, Alexandra Tolstikova, Thomas A. White, Michael Groessler, Holger Fleckenstein, Fabian Trost, Marina Galchenkova, Yaroslav Gevorkov, Chufeng Li, Salah Awel, Ariana Peck, Lourdu P. Xavier, Miriam Barthelmess, Frank Schlünzen, Nadine Werner, Hina Andaleeb, Najeeb Ullah, Sven Falke, Bruno Alves Franca, Martin Schwinzer, Hévila Brognaro, Brandon Seychell, Henry Gieseler, Diogo Melo, Jo J. Zaitsev-Doyle, Brenna Norton-Baker, Juraj Knoska, Gisel Esperanza, Aida Rahmani Mashhour, Filip Guicking, Vincent Hennicke, Pontus Fischer, Cromarte Rogers, Diana C.F. Monteiro, Johanna Hakanpää, Jan Meyer, Heshmat Noei, Phil Gribbon, Bernhard Ellinger, Maria Kuzikov, Markus Wolf, Linlin Zhang, Xinyuanyuan Sun, Jonathan Pletzer-Zelgert, Jan Wollenhaupt, Christian Feiler, Manfred Weiss, Eike-Christian Schulz, Pedram Mehrabi, Christina Schmidt, Robin Schubert, Huijong Han, Boris Krichel, Yaiza Fernández-García, Beatriz Escudero-Pérez, Stephan Günther, Dusan Turk, Charlotte Uetrecht, Tobias Beck, Henning Tidow, Ashwin Chari, Andrea Zaliani, Matthias Rarey, Russell Cox, Rolf Hilgenfeld, Henry N. Chapman, Arwen R. Pearson, Christian Betzel, and Alke Meents, Catalytic cleavage of HEAT and subsequent covalent binding of the tetralone moiety by the SARS-CoV-2 main protease, (submitted, 2020) [bib][link]

  1. Huijong Han, Joon-Hwa Lee, and Byong-Seok Choi, The Solution Structure of DNA Decamer Duplex Containing the Dewar Product of the Thymydylyl(3‘―–5‘―)thymidine by the NMR Spectroscopy and the Full Relaxation Matrix Refinement in XVIII International Conference on Magnetic Resonance in Biological Systems (Tokyo Metropolitan University, Tokyo, Japan, August 1998) [bib]
  2. Huijong Han, Joon-Hwa Lee, and Byong-Seok Choi, The Solution Structure of DNA Decamer Duplex Containing the Dewar Product of the Thymydylyl(3‘―–5‘―)thymidine by the NMR Spectroscopy and the Full Relaxation Matrix Refinement in Abstract of the 82nd Annual Meeting of the Korean Chemical Society (Oct. 23-24, 1998) [bib]
  3. Huijong Han, Todd Funke, Melanie Priestman, and Ernst Schönbrunn, Discovery and Evaluation of Novel MurA Inhibitors in Structure Based Drug Discovery (D6) (Keystone Symposia, Whistler, British Columbia, Canada, Apr. 4-9, 2006) [bib]
  4. Huijong Han, Todd Funke, Melanie Priestman, and Ernst Schönbrunn, Discovery of Novel MurA Inhibitors in The 6th Biennial GPEN Conference (University of Kansas, Lawrence, Kansas, Oct. 25-27, 2006) [bib]
  5. Huijong Han and Ernst Schönbrunn, Molecular Modes of Action of Novel CDK2 Inhibitors in The 45th Annual MIKI Meeting (University of Kansas, Lawrence, Kansas, Apr. 13-15, 2007) [oral presentation] [bib]
  6. Huijong Han, Yasmin Guel, Inari Kursula, Jana Makroczyova, Ekaterina Round, Robin Schubert, Joachim Schulz, and Kristina Lorenzen, Sample preparation and characterization for serial femtosecond crystallography at European XFEL in 16th Conference of the Asian Crystallographic Association (National University of Singapore, Singapore, December 17-20, 2019) [bib][abstract][link]

  • Goetsch Family Scholarship
  • Full governmental scholarship for undergraduate and graduate students

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